NSI-189 Phosphate

Buy NSI-189 Phosphate Research Chemical Online – USA, Europe and Worldwide

Welcome to RChems EU – your trusted global supplier of premium research chemicals. We are proud to present NSI-189 Phosphate, a high-purity research compound originally developed by Neuralstem, Inc. for neurogenesis and neuroplasticity research. NSI-189 represents a novel chemical class – a benzylidene derivative – that has been the subject of preclinical investigations into hippocampal regeneration and cognitive enhancement. Each batch is synthesized and rigorously tested to exceed 99% purity, ensuring reproducible, publication-ready results for researchers in neuroscience, cell biology, and medicinal chemistry. Whether you need to buy NSI-189 Phosphate for neurogenesis assays, behavioral pharmacology studies, or mechanistic pathway analysis, RChems EU delivers uncompromised quality with discreet worldwide shipping.

Key Features & Benefits

• >99% Certified Purity – Every batch validated by HPLC, NMR, and mass spectrometry.
• Phosphate Salt Form – Enhanced aqueous solubility for cell culture and in vitro assays.
• Neurogenesis Research Standard – Ideal for hippocampal progenitor cell proliferation studies.
• Discreet Packaging – Plain, unmarked outer boxes with no chemical names or external identifiers.
• Fast Worldwide Delivery – Expedited shipping to USA, Canada, UK, Australia, and all EU member states.
• Vacuum-Sealed & Tamper-Evident – Protection against moisture, light, and physical damage during transit.
• Batch-Specific COA – Certificate of Analysis with chromatograms included or available upon request.
• Legal Compliance Labeling – Clear “For Research Use Only” marking to support institutional procurement.

Product Specifications

Parameter	Details
Product Name	NSI-189 Phosphate (4-((Z)-(3-butylamino-4-methylphenyl)methylene)benzoic acid phosphate)
CAS Number	1270138-41-6 (phosphate salt) / 1270138-40-5 (free base)
Purity (HPLC)	≥99.0%
Form	Crystalline phosphate salt
Molecular Weight (Phosphate)	436.42 g/mol
Recommended Storage	15–25°C, sealed, away from light, desiccant recommended
Shipping Stability	30 days at ambient temperature (vacuum-sealed foil with desiccant)

Applications & Usage – For Research Only

NSI-189 Phosphate for research is strictly intended for in vitro and ex vivo laboratory applications, including but not limited to:

• Neurogenesis Assays – Quantifying hippocampal progenitor cell proliferation in primary neural stem cell cultures (e.g., adult rat hippocampus).
• Neuroplasticity Research – Studying synaptic remodeling, dendritic arborization, and spine density in rodent neuronal cultures.
• Behavioral Pharmacology – Investigating effects on cognitive function in rodent models (e.g., novel object recognition, Morris water maze, forced swim test).
• Mechanistic Pathway Analysis – Elucidating involvement of BDNF (brain-derived neurotrophic factor), TrkB signaling, and CREB phosphorylation.
• Cytotoxicity & Viability Studies – Assessing safety profiles in neuronal cell lines (e.g., SH-SY5Y, PC12, primary cortical neurons).
• Mitochondrial Function Assays – Evaluating effects on ATP production, oxidative stress, and mitochondrial biogenesis.
• Inflammatory Marker Analysis – Quantifying cytokine expression (IL-6, TNF-α, IL-1β) in microglial cell cultures.

Critical Note: This product is not for human or animal consumption, nor for therapeutic, diagnostic, or veterinary use. No in vivo administration is permitted without explicit institutional ethics approval. NSI-189 is an investigational compound with no regulatory approval anywhere in the world for any medical use.

Purity & Testing

At RChems EU, analytical rigor defines our quality. Every batch of NSI-189 Phosphate undergoes a comprehensive multi-tiered testing protocol:

1. High-Performance Liquid Chromatography (HPLC-DAD) – Quantifies main compound purity (≥99.0% area normalization) and detects related substances (impurities <1%).
2. Nuclear Magnetic Resonance (¹H NMR & ¹³C NMR) – Confirms structural identity, including the Z-configuration of the benzylidene double bond and the butylamino side chain.
3. Mass Spectrometry (LC-MS/MS) – Validates molecular weight (436.42 g/mol for phosphate salt) and assesses impurity profiles at ppm levels.
4. Residual Solvent Analysis (GC-FID) – Ensures compliance with ICH Q3C guidelines for Class 2 and Class 3 solvents.
5. Loss on Drying & Residue on Ignition – Confirms absence of excess moisture and inorganic contaminants.
6. Phosphate Content Titration – Verifies stoichiometry of the phosphate salt.
7. Heavy Metals Testing (ICP-MS) – Ensures levels below USP <232> thresholds.

A Certificate of Analysis (COA) with raw chromatograms, NMR spectra, and mass spec data is included with every shipment. Only batches meeting 99%+ purity are released. This level of purity is critical for neurogenesis research – impurities at even 1-2% can activate or inhibit off-target pathways (e.g., inflammatory cytokines or oxidative stress markers), leading to false conclusions about NSI-189’s mechanism of action. Our 99%+ guarantee ensures that observed effects are attributable to the compound itself.

Safety & Handling Guidelines

WARNING: FOR RESEARCH USE ONLY. NOT FOR HUMAN OR ANIMAL CONSUMPTION.

• Personal Protective Equipment (PPE): Always wear nitrile gloves, chemical safety goggles, and a lab coat when handling powder.
• Ventilation: Handle inside a certified chemical fume hood to prevent inhalation of airborne particulates.
• Spill Management: Gently sweep or vacuum with HEPA filter. Avoid generating dust. Dispose of as hazardous chemical waste per local regulations.
• Storage: Keep original container tightly closed at room temperature (15–25°C), away from strong oxidizers and direct sunlight. Desiccant recommended due to hygroscopic nature.
• Incompatibilities: Avoid contact with strong acids, strong bases, and oxidizing agents.
• First Aid (Accidental Exposure): Rinse exposed skin or eyes with copious water for 15 minutes. If inhaled, move to fresh air. If ingested (accidentally), seek immediate medical attention – present the Safety Data Sheet (SDS).

Global Shipping & Discreet Delivery

RChems EU specializes in fast worldwide delivery without compromising your privacy or research security.

• Plain Outer Packaging: Neutral corrugated box or padded envelope – no logos, no “NSI-189” text, no chemical names.
• Unmarked Customs Labels: Generic declaration such as “Laboratory Reference Samples – Non-Hazardous” or “Research Materials Only – No Commercial Value.”
• Inner Vacuum-Sealed Foil: Powder is contained in a heat-sealed, opaque, moisture-proof pouch with desiccant.
• Tamper-Evident Seals: Each inner pouch is heat-sealed with a unique batch number visible only upon opening.
• Tracking & Insurance: Every order receives a unique tracking number. Optional shipping insurance covers loss or damage in transit.
• Typical Transit Times (business days):
  • USA & Canada: 5–9 days
  • United Kingdom & Europe: 3–7 days
  • Australia & New Zealand: 7–12 days
  • Rest of world: 10–15 days

No signature required for standard delivery unless requested. Credit card and cryptocurrency statements show a neutral, non-chemical identifier.

Why Choose RChems EU?

• Verified Global Supplier – Thousands of successful research orders shipped to universities, neuroscience labs, and private research institutions since 2017.
• Stealth Packaging Experts – Our discreet delivery protocol maintains a 99.5% customs clearance rate across five continents.
• Real-Time Customer Support – Email or live chat response within 12 hours (including weekends and holidays).
• Bulk & Repeat Order Discounts – Contact us for tiered pricing on quantities of 500mg, 1g, 2g, 5g, and 10g.
• Flexible Payments – Accepting BTC, ETH, USDT, and major credit cards with privacy protection.
• No Medical Claims – We strictly supply research chemicals; no misleading “human consumption” or “supplement” language.
• Batch Traceability – Every order is linked to a specific batch with retained samples for one year.

Legal & Safety Disclaimer

By purchasing NSI-189 Phosphate from RChems EU, you acknowledge and agree to the following:

• You are at least 18 years of age (21 in jurisdictions where required for research chemicals).
• The product will be used exclusively for legitimate research purposes in a properly equipped laboratory setting (in vitro or ex vivo only unless licensed for in vivo).
• You have obtained any necessary local, state, and federal licenses required to possess this research chemical.
• You understand that NSI-189 is not approved by the FDA, EMA, MHRA, or any other health regulatory agency for any use. It is an investigational research chemical with no approved medical indications.
• Compliance with all applicable import, export, and possession laws is solely your responsibility.
• RChems EU reserves the right to cancel any order from jurisdictions where import is explicitly prohibited or where the buyer cannot provide proof of research affiliation upon request.
• No product sold is intended to diagnose, treat, cure, or prevent any disease or condition in humans or animals.

FAQ Section

1. What is NSI-189 Phosphate used for in research?

NSI-189 Phosphate for research is primarily used in neurogenesis and neuroplasticity studies. Original preclinical research (Neuralstem, Inc.) demonstrated that NSI-189 stimulates hippocampal progenitor cell proliferation in vitro and in vivo. Current research applications include: (1) quantifying neural stem cell proliferation via BrdU/EdU incorporation, (2) studying BDNF-TrkB-CREB signaling pathways, (3) evaluating effects on dendritic complexity and spine density, (4) behavioral pharmacology in rodent models of cognitive impairment, and (5) mitochondrial function assays in neuronal cell lines. No human or veterinary applications are permitted.

2. Is NSI-189 Phosphate legal to buy and import?

NSI-189 is not a controlled substance in most countries (USA, UK, EU, Canada, Australia) as of 2025. However, it is an unapproved investigational research chemical – its import, possession, and use are permitted only for legitimate laboratory research. It is not legal for human consumption, dietary supplement use, or self-administration in any jurisdiction. Some countries may regulate it under analogue acts if intended for human consumption (which is strictly prohibited). You are solely responsible for verifying local laws before ordering. RChems EU may request documentation of research status or institutional affiliation.

3. How discreet is your shipping?

Extremely discreet. Your order arrives in a plain, unmarked outer box with no mention of “NSI-189,” “neurogenesis,” “research chemical,” or “RChems EU” on the exterior. The customs declaration reads “Laboratory samples – Non-hazardous” or “Research materials – No commercial value.” Inside, the powder is vacuum-sealed in opaque foil with desiccant to prevent X-ray detection and moisture damage. No external markings suggest pharmaceutical or supplement content.

4. What is the guaranteed purity level, and how is it tested?

All NSI-189 Phosphate batches are independently tested and guaranteed at ≥99.0% purity via HPLC-DAD (area normalization method). Confirmation includes ¹H NMR and ¹³C NMR (structural identity, confirming Z-configuration and butylamino side chain), LC-MS/MS (molecular weight 436.42 g/mol for phosphate salt), residual solvent analysis (GC-FID), phosphate content titration, and heavy metals testing (ICP-MS). A batch-specific Certificate of Analysis (COA) with chromatograms and NMR spectra is included inside the discreet package or available digitally before purchase.

5. What if my package is seized by customs?

While our discreet packaging and compliant documentation keep seizure rates below 1% for most countries, RChems EU cannot guarantee delivery due to varying customs enforcement. If a package is officially seized and you provide a copy of the customs seizure notice, we offer a one-time reshipment at 50% of the order value (or full reshipment if you purchased shipping insurance at checkout – typically 10% of order value). Refunds are not issued for seizures due to legal compliance reasons, as all shipments are properly declared as research samples.

6. How should I store NSI-189 Phosphate powder after opening?

Store in the original, tightly closed container at room temperature (15–25°C) , away from light, moisture, and heat. NSI-189 Phosphate is hygroscopic – keep desiccant inside and minimize exposure to humid air. For long-term storage (>6 months), store under inert atmosphere (argon or nitrogen) at 2–8°C. Do not freeze-thaw repeatedly. Once opened, use within 12 months for analytical purposes. Avoid repeated opening and closing of the container.

7. What is the significance of the phosphate salt form?

NSI-189 is supplied as the phosphate salt, which offers several advantages for research: (1) Superior aqueous solubility compared to the free base – the phosphate salt dissolves readily in water, cell culture media (DMEM, RPMI), and phosphate-buffered saline (PBS) at concentrations up to 50 mg/mL. (2) Buffering capacity – the phosphate counterion helps maintain physiological pH when dissolved. (3) Improved stability – the salt form is less hygroscopic than the free base. When calculating concentrations for cell culture work, use the molecular weight of the phosphate salt (436.42 g/mol).

8. What is the mechanism of action for NSI-189 in research?

The precise molecular mechanism of NSI-189 remains under investigation. Published preclinical research suggests: (1) increased expression of BDNF (brain-derived neurotrophic factor) and TrkB receptor activation, (2) enhanced CREB (cAMP response element-binding protein) phosphorylation, (3) stimulation of mitochondrial function and ATP production, and (4) modulation of neuroinflammatory markers. However, the primary molecular target has not been definitively identified. Researchers use NSI-189 as a tool compound to study downstream neuroplasticity pathways independent of traditional receptor targets.

9. Can I order NSI-189 Phosphate in larger quantities?

Yes. For research programs requiring more than the standard quantity, contact RChems EU directly via the website contact form. We offer bulk configurations of 500mg, 1g, 2g, 5g, and 10g (same 99%+ purity) with volume-based pricing (typically 10–30% discount). Larger orders receive enhanced discreet packaging with additional tamper-evident seals, priority tracking, and expedited handling.

10. What analytical methods are recommended for NSI-189 quantification?

For researchers developing analytical methods: typical HPLC conditions include a C18 column (150 mm × 4.6 mm, 5 µm), mobile phase of acetonitrile:0.1% formic acid in water (35:65 v/v), flow rate 1.0 mL/min, UV detection at 270 nm. Retention time for NSI-189 is approximately 6.2 minutes under these conditions. For LC-MS/MS, use positive ion mode (ESI+) with MRM transition m/z 339.2 → 228.1 (free base fragment). A validated method summary is included with the COA.

11. Is NSI-189 related to other neurogenic compounds?

NSI-189 is structurally distinct from traditional neurotrophins (BDNF, NGF), nootropics (racetams), or antidepressants (SSRIs, ketamine). It belongs to the benzylidene derivative class and was discovered through phenotypic screening for hippocampal neurogenesis. Unlike BDNF (a protein that cannot cross the blood-brain barrier), NSI-189 is a small molecule that has demonstrated oral bioavailability in preclinical studies. Researchers use it as a unique pharmacological tool to study neurogenesis-independent mechanisms of cognitive enhancement.

Ready to advance your neuroscience research with certified NSI-189 Phosphate?
Buy NSI-189 Phosphate from RChems EU today and experience 99% purity, discreet worldwide delivery, and professional support – from a supplier researchers trust worldwide.

This description is for informational purposes only. No claims of safety, efficacy, or legality are made for human or animal use. Always consult your institutional review board, legal counsel, and local laws before purchasing research chemicals.