4-HO-DET fumarate

Buy 4-HO-DET Fumarate Research Chemical Online – USA, Europe & Worldwide

Among the historically significant 4-substituted tryptamines, 4-HO-DET fumarate (4-hydroxy-N,N-diethyltryptamine fumarate) occupies a notable position. First synthesized and investigated in the mid-20th century, this compound—often referred to in the scientific literature by its colloquial designation “Ethocybin”—is a structural analog of psilocin (4-HO-DMT), differing by the replacement of both methyl groups on the terminal amine with ethyl groups. This symmetric diethyl substitution pattern (C2/C2) creates a molecule with distinct steric and lipophilic properties compared to its dimethyl counterpart.

The 4-hydroxy group places 4-HO-DET within the same structural family as the active metabolite of psilocybin, making it a valuable reference compound for SAR studies. For researchers investigating structure-activity relationships within the tryptamine family, 4-HO-DET fumarate offers a well-documented tool for understanding how symmetric N-alkyl chain lengthening—from methyl to ethyl—impacts serotonin receptor binding affinity, selectivity, intrinsic activity, and metabolic stability. When you buy 4-HO-DET fumarate from RChems EU, you acquire a high-purity research compound manufactured under strict quality controls. Our 99% pure 4-HO-DET fumarate is intended exclusively for laboratory research—not for human or animal consumption—enabling precise, reproducible experiments in receptor pharmacology, neurochemistry, and SAR studies.

Key Features & Benefits

• Verified 99%+ Purity: Every batch undergoes independent HPLC, LC-MS, and ¹H/¹³C NMR analysis to guarantee ≥99% purity.
• 4-Hydroxy Substitution: The 4-hydroxy group enables comparative SAR studies across the 4-hydroxytryptamine family, including structural analogs of psilocin.
• Symmetric N,N-Diethyl Substitution: The diethyl pattern (C2/C2) allows systematic comparison with 4-HO-DMT (dimethyl, C1/C1), 4-HO-MPT (methyl/propyl, C1/C3), and 4-HO-DPT (dipropyl, C3/C3).
• Well-Documented Research History: Extensively characterized in peer-reviewed literature with abundant reference data for assay development.
• Fumarate Salt Form: Enhanced crystalline stability and predictable solubility in organic solvents compared to freebase forms.
• Receptor Selectivity Research Tool: Ideal for investigating how symmetric N-alkyl chain lengthening influences 5-HT2A vs. 5-HT1A vs. 5-HT2C receptor binding.
• Worldwide Delivery: Fast, tracked shipping to the USA, Canada, Europe, Australia, and select Asian research hubs.
• Discreet Packaging Protocol: Plain outer boxes, unmarked labels, vacuum-sealed inner bags—no chemical names on the parcel.

Product Specifications

Applications & Usage

⚠️ Strictly for laboratory research use only. Not for human or animal consumption.

4-HO-DET fumarate is utilized in a range of non-clinical, pre-clinical, and basic science research applications. Typical areas of investigation include:

• Serotonin Receptor Binding Assays: Evaluating affinity (Ki) for 5-HT1A, 5-HT2A, 5-HT2C, and other serotonin receptor subtypes using radioligand displacement (e.g., [³H]ketanserin for 5-HT2A, [³H]8-OH-DPAT for 5-HT1A, [³H]mesulergine for 5-HT2C).
• G-protein Activation Functional Studies: Measuring [³⁵S]GTPγS binding or BRET-based biosensors to assess efficacy and potency at serotonin receptors in transfected cell lines (e.g., HEK-293 cells expressing human 5-HT2A receptors).
• Symmetric N-Alkyl Chain Length SAR Investigations: Systematically comparing 4-HO-DET (diethyl, C2/C2) with 4-HO-DMT (dimethyl, C1/C1), 4-HO-DPT (dipropyl, C3/C3), and 4-HO-DiPT (diisopropyl, branched C3/C3) to map the impact of symmetric N-alkyl chain lengthening and branching on receptor selectivity, intrinsic activity, and metabolic stability.
• 4-Hydroxy Tryptamine Family Comparisons: Comparing 4-HO-DET with other 4-substituted tryptamines such as 4-HO-MET (methyl/ethyl), 4-HO-MPT (methyl/propyl), and 4-HO-EPT (ethyl/propyl) to understand the effects of symmetric vs. asymmetric substitution.
• Metabolic Stability Research: Evaluating phase I and phase II metabolism using hepatic microsomes or S9 fractions with LC-MS/MS quantification, particularly glucuronidation of the 4-hydroxy group and N-dealkylation pathways.
• Analytical Reference Standard: Serving as a high-purity calibrant for forensic toxicology, wastewater analysis, or novel psychoactive substance (NPS) screening methods.
• In Vitro Neurotoxicity Screening: Assessing cytotoxicity and oxidative stress markers in neuronal cell cultures (e.g., SH-SY5Y, primary cortical neurons).

Researchers are encouraged to consult peer-reviewed literature (e.g., Journal of Medicinal Chemistry, Neuropharmacology, ACS Chemical Neuroscience, Psychopharmacology) for established in vitro protocols. RChems EU does not provide research protocols but guarantees the compound’s structural integrity and purity for such applications.

Purity & Testing

Reproducibility in serotonin receptor research depends critically on compound purity. Impurities—particularly other 4-hydroxylated tryptamine isomers, N-desalkyl byproducts, or oxidative degradation products (the 4-hydroxy group is susceptible to oxidation)—can produce false-positive signals in radioligand binding assays or confound functional response curves. That is why 99% pure 4-HO-DET fumarate from RChems EU is subjected to a multi-laboratory quality control workflow:

1. High-Performance Liquid Chromatography (HPLC-DAD): Quantitative analysis at 220 nm, 254 nm, and 280 nm (indole ring absorption). Special attention is given to detecting oxidative degradation products. Minimum acceptance criterion: ≥99.0% peak area purity.
2. Liquid Chromatography-Mass Spectrometry (LC-MS): Confirms molecular ion [M+H]⁺ = 233.2 for the freebase and detects trace-level organic impurities down to 0.05%, including potential oxidation products.
3. Nuclear Magnetic Resonance (NMR): Both proton (¹H) and carbon-13 (¹³C) NMR verify the correct indole ring system, 4-hydroxy substitution (characteristic downfield OH proton exchangeable with D₂O), and symmetric N,N-diethyl substitution pattern (two overlapping ethyl triplets and quartets). Any spectral deviation—particularly signals indicative of degradation or incorrect N-alkyl ratios—triggers batch rejection.
4. Residual Solvent Analysis (GC-MS): Ensures compliance with ICH limits for Class 2 and Class 3 solvents.
5. Heavy Metal Testing (ICP-MS): Confirms levels below 20 ppm for lead, arsenic, cadmium, and mercury.

Each batch is assigned a unique lot number, and a Certificate of Analysis (COA) is available upon request. This rigorous testing ensures that when you buy 4-HO-DET fumarate for research, you receive a compound that performs consistently from order to order.

Safety & Handling Guidelines

⚠️ FOR LABORATORY RESEARCH USE ONLY. NOT FOR HUMAN OR ANIMAL CONSUMPTION.

• Personal Protective Equipment (PPE): Always wear nitrile gloves, a lab coat, and chemical splash goggles when handling 4-HO-DET fumarate powder.
• Engineering Controls: Use a certified chemical fume hood or glove box to prevent inhalation of airborne particulates.
• First Aid Measures:
  • Inhalation: Remove to fresh air. If breathing difficulty persists, seek medical attention.
  • Skin Contact: Wash with soap and copious water for 15 minutes. Remove contaminated clothing.
  • Eye Contact: Rinse cautiously with water for 15 minutes. Consult an ophthalmologist if irritation persists.
  • Ingestion: Do NOT induce vomiting. Rinse mouth and seek immediate medical attention.
• Spill Management: Vacuum or sweep gently (avoid generating dust). Collect in a sealed, labeled container for hazardous waste disposal.
• Storage: Store in a tightly sealed, amber glass vial under refrigeration (4°C) to minimize oxidative degradation of the 4-hydroxy group. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation. Avoid prolonged exposure to air, light, and heat.

By purchasing from RChems EU, you confirm that you are a qualified researcher (or authorized agent) and that your facility can handle this compound safely.

Global Shipping & Discreet Delivery

RChems EU has perfected a discreet, reliable global logistics system for all research chemical orders, including 4-HO-DET fumarate. We understand that confidentiality is essential for many academic and private research organizations.

What discreet delivery means for you:

• Plain Outer Packaging: Your order arrives in a non-descript padded envelope or small cardboard box. No logos, no brand names, no chemical identifiers.
• Unmarked Shipping Label: The return address uses a generic name (e.g., “L.C. Distribution” or “WH Logistics”). No reference to “RChems EU” or “4-HO-DET fumarate.”
• Vacuum-Sealed Inner Bags: Each bag is heat-sealed to prevent leakage, odor, and tampering. No chemical name appears on the inner bag.
• No External Documentation: Invoices, COAs, or packing slips are sent via encrypted email only—never placed inside the parcel.

Fast worldwide delivery times (tracked):

Every shipment includes a real-time tracking number. Expedited courier options (FedEx Priority, DHL Express) are available at checkout for faster delivery (2–5 days globally).

Why Choose RChems EU?

• Stealth Packaging Experts: Over 7,000 successful shipments to 45+ countries with minimal customs interference.
• Third-Party Verified Purity: Every batch is independently tested. We do not sell unverified or “research grade” material without documentation.
• Responsive Scientific Support: Technical inquiries answered within 24 hours. Replacement policy for lost or damaged shipments (terms apply).
• No Minimum Order Quantity (MOQ): Order 0.25 grams or 5 grams—same meticulous packaging and quality standards.
• Flexible Payment Methods: Secure cryptocurrency (BTC, ETH, USDT) and select credit card options.
• Global Compliance Awareness: We monitor international regulations and adjust shipping policies accordingly.

Legal & Safety Disclaimer

⚠️ IMPORTANT – READ CAREFULLY BEFORE PURCHASING

4-HO-DET fumarate (4-hydroxy-N,N-diethyltryptamine fumarate) is sold by RChems EU strictly as a research chemical and is not for human consumption, animal testing, or any form of in vivo use without appropriate institutional ethical approval. This product is not a pharmaceutical, medicinal product, dietary supplement, or recreational substance.

Legal Status Varies by Jurisdiction: 4-HO-DET fumarate may be a controlled substance or regulated analog in certain countries, states, or territories. In the United States, it may be considered a controlled substance analogue under the Federal Analogue Act (21 U.S.C. § 813) if intended for human consumption. Several European countries (including Germany, Sweden, and the UK) have specific analogue legislation or blanket scheduling for tryptamine derivatives. It is your sole responsibility to verify the legal status of 4-HO-DET fumarate in your country, state, or province before ordering.

By completing your purchase, you certify that:

1. You are at least 18 years of age.
2. You are a qualified researcher affiliated with a bona fide laboratory or academic institution.
3. You will use this product exclusively for lawful, laboratory-based research purposes.
4. You will not introduce this compound into the body of any human or animal.

RChems EU reserves the right to cancel any order that appears destined for non-research use or for a jurisdiction where 4-HO-DET fumarate is prohibited.

FAQ Section

1. What is 4-HO-DET fumarate used for in research?

4-HO-DET fumarate (4-hydroxy-N,N-diethyltryptamine fumarate) is used in neuropharmacological research to study serotonin receptor binding affinity (5-HT2A, 5-HT1A, 5-HT2C), the effects of symmetric N-alkyl chain lengthening on 4-hydroxytryptamine pharmacology, and structure-activity relationships comparing 4-HO-DET with psilocin (4-HO-DMT) and other 4-substituted tryptamines. It is particularly valuable for systematic SAR studies of symmetric N-alkyl chain progression from methyl to ethyl to propyl.

2. Is 4-HO-DET fumarate legal to buy and possess for research?

Legality depends on your jurisdiction. 4-HO-DET is not explicitly scheduled at the US federal level but may be considered a controlled substance analogue under the Federal Analogue Act (21 U.S.C. § 813) if intended for human consumption. Many European countries have analogue legislation or specific tryptamine scheduling. You must check your local regulations before ordering. RChems EU ships on the condition that you assume full legal responsibility.

3. How discreet is the shipping from RChems EU?

Extremely discreet. All 4-HO-DET fumarate orders are shipped in plain, unmarked packaging with no external branding. The shipping label uses a generic return address. Inner bags are vacuum-sealed with no chemical names or CAS numbers visible. Our stealth protocol has enabled successful deliveries to over 45 countries with a 99%+ success rate.

4. What is the guaranteed purity level of your 4-HO-DET fumarate?

Every batch of 4-HO-DET fumarate from RChems EU is independently tested to be ≥99% pure via HPLC-DAD, with confirmation by LC-MS and ¹H/¹³C NMR. Special attention is given to detecting oxidative degradation products of the 4-hydroxy group. A lot-specific Certificate of Analysis (COA) is available for every order upon request.

5. How is the 99% purity tested?

We employ a five-tier analytical protocol:

• HPLC-DAD for quantitative purity (≥99% peak area at 220 nm, 254 nm, and 280 nm), with methods optimized to detect oxidation products.
• LC-MS for molecular weight confirmation ([M+H]⁺ = 233.2 for freebase) and trace impurity detection down to 0.05%.
• ¹H and ¹³C NMR for structural confirmation (indole ring system, 4-hydroxy substitution with exchangeable proton, symmetric N,N-diethyl substitution pattern).
• GC-MS for residual solvent analysis (ICH compliance).
• Heavy metal testing (ICP-MS) to confirm levels below 20 ppm.

All testing is performed by an ISO 17025-accredited contract laboratory.

6. Can you ship 4-HO-DET fumarate to my country (USA, UK, Germany, Australia, Canada)?

RChems EU ships 4-HO-DET fumarate worldwide, including the USA, Canada, United Kingdom, Germany, France, Italy, Spain, Netherlands, Switzerland, Norway, Australia, and New Zealand. However, due to varying legal statuses of tryptamine derivatives, we strongly recommend contacting our support team before ordering if you are located in Germany, Sweden, Finland, or any country with broad analogue legislation. For countries with strict import controls (e.g., Japan, Singapore, South Korea), we do not currently ship 4-HO-DET fumarate.

7. How should I store 4-HO-DET fumarate after delivery?

Store 4-HO-DET fumarate in a tightly sealed, amber glass vial under refrigeration (4°C) to minimize oxidative degradation of the 4-hydroxy group. For short-term use (≤2 weeks), room temperature (20–25°C) in a desiccator under inert atmosphere (argon or nitrogen) may be acceptable. Always allow the sealed container to reach room temperature before opening to prevent condensation. Avoid prolonged exposure to air, light, and heat.

8. What is the difference between 4-HO-DET and psilocin (4-HO-DMT)?

The key structural difference lies in the N-alkyl substitution pattern:

• Psilocin (4-HO-DMT) has two methyl groups (N,N-dimethyl) – C1/C1, both chains one carbon
• 4-HO-DET has two ethyl groups (N,N-diethyl) – C2/C2, both chains two carbons

In serotonin receptor research, extending both alkyl chains from methyl to ethyl significantly increases lipophilicity, alters receptor binding affinity, changes selectivity for 5-HT2A vs. 5-HT1A vs. 5-HT2C, and modifies metabolic stability. Comparing 4-HO-DET with psilocin allows researchers to map the impact of symmetric N-alkyl chain lengthening on 4-hydroxytryptamine pharmacology.

9. What is the difference between 4-HO-DET and 4-HO-DPT?

The difference lies in the length of the N-alkyl chains:

• 4-HO-DET has two ethyl groups (N,N-diethyl) – C2/C2, chains of two carbons each
• 4-HO-DPT has two propyl groups (N,N-dipropyl) – C3/C3, chains of three carbons each

Comparing these two compounds allows researchers to investigate the effects of increasing symmetric N-alkyl chain length from ethyl (C2) to propyl (C3). This provides a focused SAR study on how adding one methylene unit to each alkyl chain influences receptor binding, selectivity, and functional activity.

10. What is the difference between 4-HO-DET and 4-HO-MET?

The difference lies in the symmetry of the N-alkyl substitution:

• 4-HO-MET has one methyl and one ethyl group (N-methyl-N-ethyl) – asymmetric (C1/C2)
• 4-HO-DET has two ethyl groups (N,N-diethyl) – symmetric (C2/C2)

Comparing these two compounds allows researchers to investigate the effects of symmetric vs. asymmetric N-alkyl substitution. Both have a combined alkyl chain length of 3 carbons for 4-HO-MET (1+2) and 4 carbons for 4-HO-DET (2+2), so this comparison also involves total carbon length differences.

11. Do you offer a reshipment policy if customs seizes my package?

Yes. RChems EU offers a one-time reshipment policy for confirmed customs seizures in most countries, provided that the original shipping address is valid and the buyer did not violate known local laws. Crypto payments qualify for the full reshipment guarantee. Terms and conditions apply—please contact customer support for details.

12. Can I get a Certificate of Analysis (COA) before ordering?

Absolutely. Contact our support team via email with the product name (4-HO-DET fumarate or 4-hydroxy-N,N-diethyltryptamine fumarate) and your research affiliation. We will provide a redacted or full COA for the current batch, including HPLC chromatograms and NMR spectra, subject to verification of legitimate research intent.

Ready to advance your symmetric N-alkyl 4-hydroxytryptamine research? Order high-purity 4-HO-DET fumarate from RChems EU today and experience unmatched product quality, stealth global delivery, and professional scientific support. Buy 4-HO-DET Fumarate for Research

RChems EU – Your trusted global supplier of 99% pure research chemicals.